Reverse Genetics and Its Application in Research of Classical swine fever virus

Classical swine fever is one of the most serious diseases of swine. Its pathogen is classical swine fever virus with single chain of positive RNA. Attenuated vaccine of Classical swine fever have played an important role in the control of the disease. But its use is seriously restricted in recent years, the reason is that the vaccinated animal and the infected animal can not be distinguished by normal method. Using marker vaccine is very important to solve this problem. Virus molecular biology is a necessary method for its study. The RNA structure of Classical swine fever virus had restricted the study for a long time. The reverse genetics could translate the RNA into cDNA, it extremely promoted the study on RNA viruses. The significance of reverse genetics, its study method, the advance and its application in the study of on new vaccine were synthetically summarized in this article.