2012•Guiyang yixueyuan xuebaoRequires access

Determination of Hesperidin in Shensu Oral Liquid with HighPerformance Liquid Chromatography

Chang Ming Ling

Abstract

Objective: To establish a method for determination of hesperidin in Shensu oral liquid with high performance liquid chromatography(HPLC).Methods: HPLC was carried out on Waters-symmetry C18(WAT054275,5 μm,250 mm×4.6 mm) column with methyl alcohol-acetic acid-water(35∶4∶61) as mobile phase,and the flow rate was 1 ml/min.Detecion wavelength was 283 nm.Results: The calibration curve for hesperidin was linear in the range of 0.073 56～1.103 4 μg(r=0.999 9).The average recovery was 96.91%～98.31%,and RSD% was 0.53%(n=6).Conclusions: This method is simple,quick,accurate and reproducible for quality control of Shensu oral liquid.

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Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Calibration curve, Chemistry, Acetic acid, Detection limit, Medicine