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2010•The Chinese Journal of Modern Applied PharmacyRequires access

HPLC Determination of Contents and Related Substances of Synthetic Thymosin α_1

Su Min

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Abstract

OBJECTIVE To assay the content and related substances of thymosin α1.METHODS HPLC was exploited to determinate the content and related substances of thymosin α1 at 215 nm.The separation was performed on Vydac C18 column(250 mm×4.6mm,5 μm) with the mobile phase of 0.02 mol·L-1 phosphate buffer(pH 7.00)-isopropanol(95∶5) under 25 ℃ .RESULTS The linearity was obtained over the range of 0.01-0.83 mg·mL-1(r=0.999 8).The LOD was 10 ng.Related substances were 2.5%?2.6%.CONCLUTION The method is simple,convenient,accurate and can be employed to assay the content and related substances of thymosin α1 for its quality control.

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OBJECTIVE To assay the content and related substances of thymosin α1.METHODS HPLC was exploited to determinate the content and related substances of thymosin α1 at 215 nm.The separation was performed on Vydac C18 column(250 mm×4.6mm,5 μm) with the mobile phase of 0.02 mol·L-1 phosphate buffer(pH 7.00)-isopropanol(95∶5) under 25 ℃ .RESULTS The linearity was obtained over the range of 0.01-0.83 mg·mL-1(r=0.999 8).The LOD was 10 ng.Related substances were 2.5%?2.6%.CONCLUTION The method is simple,convenient,accurate and can be employed to assay the content and related substances of thymosin α1 for its quality control.

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Available abstract

OBJECTIVE To assay the content and related substances of thymosin α1.METHODS HPLC was exploited to determinate the content and related substances of thymosin α1 at 215 nm.The separation was performed on Vydac C18 column(250 mm×4.6mm,5 μm) with the mobile phase of 0.02 mol·L-1 phosphate buffer(pH 7.00)-isopropanol(95∶5) under 25 ℃ .RESULTS The linearity was obtained over the range of 0.01-0.83 mg·mL-1(r=0.999 8).The LOD was 10 ng.Related substances were 2.5%?2.6%.CONCLUTION The method is simple,convenient,accurate and can be employed to assay the content and related substances of thymosin α1 for its quality control.

Key concepts: Thymosin, Chromatography, Chemistry, High-performance liquid chromatography, Phosphate buffered saline, Biochemistry

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