ScholarLens Back to search
2004•Zhongguo sheng-hua yaowu zazhiRequires access

Determination of related substances of cAMP by RP-Ion-Pair chromatography

Shi Bei-jia

Open publisher page 0 citations

Abstract

PurposeTo establish an HPLC method for determination of cAMP′s related substances.MethodsAn optimal HPLC method was set up. The samples were separated on a C 18 column using 0.05 mol/L KH 2PO 4(containing 0.01mol/L TBA,pH 4.3)-acetonitrile(85∶15)as mobile phase and detected at 259 nm. The flow rate was 1.0 ml/min. ResultsAdenosin, AMP, cAMP and unknown substance were well separated. Separation factors were 9.2, 11.5 and 14.3 respectively. The detection limits of Adenosin and AMP were both 0.1 ng, and the determination limits were both 0.3 ng.ConclusionThis method was convenient, reliable and sensitive. It was superior to paper chromatography.

About this research paper

What this paper is about

PurposeTo establish an HPLC method for determination of cAMP′s related substances.MethodsAn optimal HPLC method was set up. The samples were separated on a C 18 column using 0.05 mol/L KH 2PO 4(containing 0.01mol/L TBA,pH 4.3)-acetonitrile(85∶15)as mobile phase and detected at 259 nm. The flow rate was 1.0 ml/min. ResultsAdenosin, AMP, cAMP and unknown substance were well separated. Separation factors were 9.2, 11.5 and 14.3 respectively. The detection limits of Adenosin and AMP were both 0.1 ng, and the determination limits were both 0.3 ng.ConclusionThis method was convenient, reliable and sensitive. It was superior to paper chromatography.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

PurposeTo establish an HPLC method for determination of cAMP′s related substances.MethodsAn optimal HPLC method was set up. The samples were separated on a C 18 column using 0.05 mol/L KH 2PO 4(containing 0.01mol/L TBA,pH 4.3)-acetonitrile(85∶15)as mobile phase and detected at 259 nm. The flow rate was 1.0 ml/min. ResultsAdenosin, AMP, cAMP and unknown substance were well separated. Separation factors were 9.2, 11.5 and 14.3 respectively. The detection limits of Adenosin and AMP were both 0.1 ng, and the determination limits were both 0.3 ng.ConclusionThis method was convenient, reliable and sensitive. It was superior to paper chromatography.

Key concepts: Chromatography, Chemistry, High-performance liquid chromatography, Detection limit, Acetonitrile, Ion chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of related substances of cAMP by RP-Ion-Pair chromatography — Research Paper | ScholarLens