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2009•Acta Academiae Medicinae ZunyiRequires access

Cultural method of dorsal root ganglion neurons of adult rat for patch clamp study

Huai‐Zhen Ruan

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Abstract

Objective To establish a kind of cultural method of dorsal root ganglion (DRG) neurons for patch clamp study. Methods DRG neurons of adult rat were isolated and cultured. The electrophysiological properties and ATP-induced currents of cultured DRG neurons were investigated by using patchclamp techniques. Results The cultured cells were morphologically intact,from which evoked action potential and Na+ channel currents,and ATP-induced currents could be detected. Conclusions Cultured DRG neurons of adult rat are ideal for patch-clamp studies.

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Objective To establish a kind of cultural method of dorsal root ganglion (DRG) neurons for patch clamp study. Methods DRG neurons of adult rat were isolated and cultured. The electrophysiological properties and ATP-induced currents of cultured DRG neurons were investigated by using patchclamp techniques. Results The cultured cells were morphologically intact,from which evoked action potential and Na+ channel currents,and ATP-induced currents could be detected. Conclusions Cultured DRG neurons of adult rat are ideal for patch-clamp studies.

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Available abstract

Objective To establish a kind of cultural method of dorsal root ganglion (DRG) neurons for patch clamp study. Methods DRG neurons of adult rat were isolated and cultured. The electrophysiological properties and ATP-induced currents of cultured DRG neurons were investigated by using patchclamp techniques. Results The cultured cells were morphologically intact,from which evoked action potential and Na+ channel currents,and ATP-induced currents could be detected. Conclusions Cultured DRG neurons of adult rat are ideal for patch-clamp studies.

Key concepts: Dorsal root ganglion, Patch clamp, Electrophysiology, Dorsum, Neuroscience, Clamp, Anatomy, Biology

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