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2014•Cold Spring Harbor ProtocolsRequires access

Whole-Cell Patch-Clamp Recordings of Ca2+ Currents from Isolated Neonatal Mouse Dorsal Root Ganglion (DRG) Neurons

María A. Gandini, Alejandro Sandoval, Ricardo Felix

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Abstract

Primary culture of sensory neurons from dorsal root ganglia (DRGs) is a widely used model for studying Ca(2+) channels. DRG neurons can be collected from neonate or adult mice; the production of cultures can take a couple of hours, and cells so derived can be used almost immediately or maintained for as long as 1 wk. This method allows the isolation of neurons for numerous experimental purposes, including whole-cell patch-clamp recording. The purpose of this protocol is to provide a description of methods commonly used for the harvest and growth of DRG neonatal neurons as well as for recording whole-cell currents through voltage-sensitive Ca(2+) channels in these cells.

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What this paper is about

Primary culture of sensory neurons from dorsal root ganglia (DRGs) is a widely used model for studying Ca(2+) channels. DRG neurons can be collected from neonate or adult mice; the production of cultures can take a couple of hours, and cells so derived can be used almost immediately or maintained for as long as 1 wk. This method allows the isolation of neurons for numerous experimental purposes, including whole-cell patch-clamp recording. The purpose of this protocol is to provide a description of methods commonly used for the harvest and growth of DRG neonatal neurons as well as for recording whole-cell currents through voltage-sensitive Ca(2+) channels in these cells.

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Available abstract

Primary culture of sensory neurons from dorsal root ganglia (DRGs) is a widely used model for studying Ca(2+) channels. DRG neurons can be collected from neonate or adult mice; the production of cultures can take a couple of hours, and cells so derived can be used almost immediately or maintained for as long as 1 wk. This method allows the isolation of neurons for numerous experimental purposes, including whole-cell patch-clamp recording. The purpose of this protocol is to provide a description of methods commonly used for the harvest and growth of DRG neonatal neurons as well as for recording whole-cell currents through voltage-sensitive Ca(2+) channels in these cells.

Key concepts: Dorsal root ganglion, Patch clamp, Neuroscience, Primary culture, Dorsum, Sensory system, Clamp, Anatomy

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Whole-Cell Patch-Clamp Recordings of Ca2+ Currents from Isolated Neonatal Mouse Dorsal Root Ganglion (DRG) Neurons — Research Paper | ScholarLens