2011•PubMedRequires access

[A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel].

Cheng-Biao Zhang, Xiaolu Yu, Zhiqiang Pan, Cuijie Shao, Jun‐Li Cao

Abstract

OBJECTIVE: To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel. METHODS: DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing. RESULTS: The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded. CONCLUSION: The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.

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Key concepts: Dorsal root ganglion, Patch clamp, Clamp, Dorsum, Current clamp, Chemistry, Ganglion, Anatomy