Establishment and identification of swine model of acute myocardial infarction by coronary balloon occlusion with PTCA technique

Objective To investigate and identify a swine model of acute myocardial infarction(AMI) by coronary occlusion with angioplasty balloon.Methods After anesthesia,eight swines underwent 90()minutes of occlusion and 60()minutes of reperfusion of the obtuse marginal branch of the left circumflex(LCX)coronary artery by inflating and deflating a transcatheter balloon.The in vivo MR images enhanced with a necrosis-avid contrast agent(EC-60) and postmortem 2,3,5-triphenyltetrazolium chloride(TTC) histochemical staining were performed to confirm AMI.Results The AMI were successfully induced in all 8 swines.The distribution and mean EC-60 induced hyperintense areas on in vivo MR images were consistent with that of AMI as identified by using TTC staining(P0.05).Conclusions A swine model of AMI by coronary occlusion with angioplasty balloon could be established effectively,which proves to be reproducible,controllable,andminimally invasive.This model mimics human conditions and should be feasible for pertinent experimental research.