Establishment of swine models of acute myocardial infarction by coronary occlusion

Objective To explore and develop swine models of acute myocardial infarction(AMI) made by coronary occlusion with angioplasty balloon in percutaneous coronary angioplasty(PTCA).Methods After anesthesia,12 swine were implanted with balloons into the branch of the left circumflex artery(LCX).The balloon was inflated and occluded the LCX for 90 min.Their electrocardiography,blood pressure and ultrasonography were monitored.In the end,the animals were killed,the hearts were stained with TTC and the histomography changes of myocardial cells were assessed by light and electron microscope.Results Twelve swine underwent successful induction of AMI.Regional wall motion abnormalities were found by two dimensional echocardiography one hour after AMI in all swine.AMI were confirmed by dynamic changes of electrocardiography,TTC staining and microscope.Conclusion A simple,rapid,and effective method of producing myocardial infarction in swine has been established without associated significant arrhythmias or mortality.