Development of acute myocardial infarction reperfusion model with Chinese mini pig by coronary occlusion with balloon angioplasty

Objective:To investigate the method of acute reperfusion myocardial infarction model with Chinese mini pigs by coronary occlusion with balloon angioplasty.Methods:Under anesthesia,distal segment of left anterior descending artery were blocked with balloon angioplasty for 90 minutes in eleven Chinese mini pigs.Then taking back the balloon,the acute reperfusion myocardial infarction models were developed.Electrocardiogram and magnetic resonance imaging(MRI) were performed to evaluate in vivo.Triphenyltetrazolium chloride(TTC) stain was performed after execution,and compared with MRI.Results:Eight models were successful,and the other three died for ventricular fibrillation(success rate was 72.73%).Electrocardiogram showed the ischemic-reperfusion injury with the elevation of ST segment.MRI displayed the volume percent of myocardial infarction was(21.6±6.1)%,and locating in apex,anterior,and anteroseptal segments.The results of TTC stain were corresponding with MRI,and the volume percent was(21.1±6.2)%.The difference between them was not significant(P0.05).Conclusion:Coronary occlusion with balloon angioplasty can develop acute reperfusion myocardial infarction model successfully,and provide the basis of experimental model for correlative investigation.