2007•Journal of Anhui Traditional Chinese Medical CollegeRequires access

Content Determination of Puerarin in Puerarin Microemulsion by HPLC

Hongfei Wu

Abstract

Objective:To establish an HPLC method for determining the content of puerarin in Puerarin Microemulsion.Methods: Shim-pack VP-ODS column(4.6 mm×150 mm) was employed,and the mobile phase consisted of methanol-1.0 g/L acid citric(25∶75).The detective wavelength was set at 250 nm.The flow rate was 0.8 ml/min,and the column temperature was at 40℃.Results: There was good resolution between puerarin and the excipients under this chromatographic condition.The calibration curve was linear in the range of 5.29～132.25 μg/ml(r=0.999 95).Conclusion: The method is simple and accurate in determining the content of puerarin in Puerarin Microemulsion.

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Key concepts: Puerarin, Chromatography, Microemulsion, High-performance liquid chromatography, Calibration curve, Chemistry, Citric acid, Content determination