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2006•Research and Practice on Chinese MedicinesRequires access

Determination of Puerarin in Puerarin Injection by HPLC

Ruan Hong-sheng

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Abstract

Objective To establish the determination method of puerarin in puerarin injection.Methods HPLC was used on ODS C_(18) column,methanol-1%glacial acetic acid(1:3) was used as a mobile phase.The detection wave-length was at 250nm.Results The detection range was 0.32~1.60ug.There was a good linear relationship between consistence and apex-area.The regress equation was Y=2E+06X+33911,r=0.999 9.The recovery of the added sample was 99.82%,and RSD=0.98%.Conclusion This method was simple and reliable,and could be used for the control quality of puerarin injection.

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What this paper is about

Objective To establish the determination method of puerarin in puerarin injection.Methods HPLC was used on ODS C_(18) column,methanol-1%glacial acetic acid(1:3) was used as a mobile phase.The detection wave-length was at 250nm.Results The detection range was 0.32~1.60ug.There was a good linear relationship between consistence and apex-area.The regress equation was Y=2E+06X+33911,r=0.999 9.The recovery of the added sample was 99.82%,and RSD=0.98%.Conclusion This method was simple and reliable,and could be used for the control quality of puerarin injection.

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Available abstract

Objective To establish the determination method of puerarin in puerarin injection.Methods HPLC was used on ODS C_(18) column,methanol-1%glacial acetic acid(1:3) was used as a mobile phase.The detection wave-length was at 250nm.Results The detection range was 0.32~1.60ug.There was a good linear relationship between consistence and apex-area.The regress equation was Y=2E+06X+33911,r=0.999 9.The recovery of the added sample was 99.82%,and RSD=0.98%.Conclusion This method was simple and reliable,and could be used for the control quality of puerarin injection.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Chemistry, Methanol, Acetic acid, Linear range, Detection limit

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