2014•China Animal Husbandry & Veterinary MedicineRequires access

Study on Expression of L-type Amino Acid Transporter 1 in Development and Lactation of Mouse Mammary Gland

Yunfeng Lin

Abstract

To investigate the underlying relationship between amino acid transporter expression and mammary gland development,the expression of LAT1and 4F2hc in virgin,pregnancy,lactation and involution of mouse mammary gland was detected by RT-PCR and laser confocal microscope.The results showed that LAT1and 4F2hc located on the membrane of ductal epithelial cell,myoepithelial cell and adipocyte in virgin mammary gland.The expression of LAT1and 4F2hc was lower because duct development was slow in this period.In pregnancy,LAT1and 4F2hc located on the basolateral of ductal epithelial cell. The expression of LAT1and 4F2hc went up as ductal epithelial cell proliferation increased.In lactation,LAT1and 4F2hc located on the basolateral of acinar epithelial cells.The expression of LAT1and 4F2hc was the highest because of milk protein synthesis.In involution,acinar structure collapsed.The expression of LAT1and 4F2hc decreased.All these results revealed that LAT1/4F2hc was an important amino acid transporter in mouse mammary gland.The expression of LAT1and 4F2hc was related to amino acid requirement in mammary gland development and lactation.

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Key concepts: Myoepithelial cell, Involution (esoterism), Mammary gland, Lactation, Biology, Amino acid transporter, Ductal cells, Internal medicine