Expression of Clusterin、Livin、Caspase-3 protein in invasive ductal breast cancer

Objective: To investigate the expression of Clusterin、 Livin、Caspase-3 protein in invasive ductal breast cancer and its clinical significance.Methods: Expression of Clusterin、Livin、Caspase-3 protein in adjacent normal breast tissue that collected from 70 cases of invisive ductal breast cancer,20 cases of breast hyperplasia,as well as 10 cases of breast cancer were evaluated with SP immunohistochemistry(IHC).The correlation among expression of the Clusterin、Livin、Caspase-3 protein and their relationship with other clinical pathological parameters were investigated.Results: Clusterin、Livin expression in breast cancer tissues were 71.4%、64.3%,significantly higher than 20%、25% in benign lesions and 0%、10% in adjacent normal tissues(P0.05);Expression of Clusterin proteins in breast cancer tissues were positively related to that of Livin proteins(P0.05).The positive expression of Caspase-3 in breast cancer tissues was 45.7%,significant lower than 75% in benign breast lesions,and 80% in adjacent tissues(P0.05).expression of Clusterin、Livin proteins in breast cancer tissues were negatively correlated with that of Caspase-3 protein(P0.05).expression of Clusterin protein expression in ductal breast cancer increased with the histologic grade and was related to lymph node metastasis status(P0.05),but it was irrelevant to the age or tumor size(P0.05);Similarly,expression of Livin protein in ductal breast cancer increased with clinical grade the but no correlation between its expression and age,tumor size,lymph node metastasis status or histologic grade was found(P0.05).Expression level of Caspase-3 protein decreased with clinical stage(P0.05),and was irrelevant to the age,tumor size,histologic grade and lymph node metastasis(P0.05).Clusterin、Livin proteins were negatively related to ER and PR in breast cancer tissues(P0.05),and was not correlated with C-erbB-2 protein(P0.05).Caspase-3 proteins were related to ER and PR in breast cancer tissues(P0.05),but uncorrelated with C-erbB-2 proteins(PD0.05).Conclusion: Clusterin、Livin may play an important role in the onset and development of breast cancer by inhibiting cell apoptosis,thus can be potential indicator for prognosis of ductal breast cancer.Besides,therapy target to both Clusterin、Livin can be new treatment for ductal breast cancer.This study also suggeste that Caspase-3 protein play an important role in the anti-apoptotic pathway of Clusterin、Livin.