Study on RAPD of Leonurus japonicus populations from Zhejiang and its adjacent areas
Jianhua Chen
Abstract
Jianhua Chen
Abstract
Seven populations of Leonurus japonicus collected from Zhejiang Province and its adjacent areas were analyzed by using random amplified polymorphic DNA (RAPD). Sixty-five bands were amplified by the 8 primers, among which 69. 23% were polymorphic. Jaccard coefficient was applied to compare the genetic similarities of these seven populations based on the RAPD data. Three-dimensional ordination plot of the seven populations based on the Jaccard coefficient was constructed by using the method of principal axes analysis (PAA). The results showed that the genetic differentiations of Leonurus japonicus are correlated to their geographical location to a certain extent.
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Seven populations of Leonurus japonicus collected from Zhejiang Province and its adjacent areas were analyzed by using random amplified polymorphic DNA (RAPD). Sixty-five bands were amplified by the 8 primers, among which 69. 23% were polymorphic. Jaccard coefficient was applied to compare the genetic similarities of these seven populations based on the RAPD data. Three-dimensional ordination plot of the seven populations based on the Jaccard coefficient was constructed by using the method of principal axes analysis (PAA). The results showed that the genetic differentiations of Leonurus japonicus are correlated to their geographical location to a certain extent.
Key concepts: Jaccard index, RAPD, Ordination, Biology, Genetics, Evolutionary biology, Traditional medicine, Genetic diversity