RAPD analysis of a population of Eremochloa ophiuroides in China
Jiping Xuan, He Gao, Jianxiu Liu
Abstract
Jiping Xuan, He Gao, Jianxiu Liu
Abstract
RAPD with 26 screened primers were conducted to assess the genetic diversity of 50 plants of 6 populations, with 158 bands amplified totally, the bands per primer were 6.08,67 bands were polymorphic and the rate was shown to be 42.41%. The results based on POPOGENE32 showed that there was significant variation amongst the populations, with about 30% and 70% genetic diversity detected amongst and within populations, respectively. Populations from Guiyang in the Guizhou province were shown to have the highest genetic diversity, with populations from Nanjing and Guangzhou shown to have the lowest genetic diversity. Dendrogram by UPGMA showed that the Nanjing and Guangzhou populations were classified together first and the population from Lianyungang in the Jiangsu province was classified last compared to the others.
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RAPD with 26 screened primers were conducted to assess the genetic diversity of 50 plants of 6 populations, with 158 bands amplified totally, the bands per primer were 6.08,67 bands were polymorphic and the rate was shown to be 42.41%. The results based on POPOGENE32 showed that there was significant variation amongst the populations, with about 30% and 70% genetic diversity detected amongst and within populations, respectively. Populations from Guiyang in the Guizhou province were shown to have the highest genetic diversity, with populations from Nanjing and Guangzhou shown to have the lowest genetic diversity. Dendrogram by UPGMA showed that the Nanjing and Guangzhou populations were classified together first and the population from Lianyungang in the Jiangsu province was classified last compared to the others.
Key concepts: RAPD, UPGMA, Genetic diversity, Dendrogram, Biology, Population, Genetic variation, Veterinary medicine