2007•Zhonghua zhongyiyao xuekanRequires access

Determination of Hesperidin in Ruyanxiao by HPLC

Yuncong C. Li

Abstract

Objective:To establish an accurate method for the determination of hesperidin in Ruyanxiao.Method:To apply an HPLC method for determination of hesperidin in Ruyanxiao.A slim-pack CLC-ODS column(6.0mm×240mm)was used with mobile phase of mathanol-0.025mol/L phosphoric acid solution(40∶60).The detection wavelength was 284nm.Results:The calibration curve was linear within the range of 0.027～0.24μg,and the average recovery of hesperidin was 97.3%(n=5),RSD=1.0%.Conclusion:The method is rapid,accurate,stability and reproductivity.It can be used for quality control of Ruyanxiao.

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Key concepts: Hesperidin, Chromatography, Calibration curve, Phosphoric acid, High-performance liquid chromatography, Chemistry, Linear range, Linear relationship