Cloning and Analysis of A New Bovine Interferon-τ Gene

[Objective] The research aimed to clone and analyze a new IFN-τ gene from yellow cattle in Shenyang area.[Method] A pair of specific primers were designed and synthesized according to the sequence(AY665673) of bovine IFN-τ gene published on NCBI website.The genomic DNA extracted from the kidney tissues of yellow cattle was taken as template for PCR amplification.After being reclaimed,the amplified products were connected with vector pUCm-T and transformed into competent cell DH5α of Escherichia coli.After the obtained plasmid was identified by PCR and double-enzyme digestion,it was made for sequencing and sequence analysis.[Result] Through PCR amplification,a specific band with the size of about 600 bp was obtained.Bovine IFN-τ gene was successfully recombined with the vector and the positive clone was obtained.The results of sequence analysis showed that the size of the cloned fragment was 586 bp and stop codon appeared at 376 bp and led to the translation termination.The nucleotide homology of this fragment with that of the sequence of AY665673 gene was 93.3% and their homology on the amino acid was 88.1%.[Conclusion] The cloned fragment was a new bovine IFN-τ gene probably.