Expression of HSP70 in early myocardial ischemia and reperfusion injury.

Objective To study the changes of the expression of HSP70 in myocardial tissue after early myocardial ischemia and reperfusion injury (MI/R), and search a new evidence for forensic pathological diagnosis in case of sudden cardiac death. Methods The rabbit model of MI/R was established, the expression of HSP70 in myocardial tissue was observe used immunohistochemical SABC method. Results The expression of HSP70 was detected in the subendothelial area of MI/R after 15 min ischemia followed by 30 min reperfusion. The positive expression of HSP70 was diffused in all myocardial tissue of MI/R after 30 min ischemia followed by 30 min reperfusion. However, the positive expression was markedly decreased in the area of MI/R but slightly increased in its margin area after 60 min ischemia followed by 30 min reperfusion. The negative staining in myocardium was found in the control group and nonischemic experimental groups. Conclusion The positive expression of HSP70 in myocardial tissue is a sensitive marker of MI/R. The detection expression of HSP70 can helpful to the postmortem diagnosis of sudden cardiac death caused by MI/R.