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The expression of TNF-α and ICAM-1 in myocardial tissue after liver ischemia-reperfusion in rats

Ya Liu

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Abstract

Objective: To observe the expression of tumor necrosis factor-α (TNF-α)and intercellular adhesion molecule-1 (ICAM-1)induced by liver ischemia/reperfusion injury in Wistar rats, and study its mechanism. Methods: A model of liver ischemia/reperfusion injury was established by imitation. Forty-eight male Wistar rats were randomly divided into 6 groups as follows: the control group (the group of sham operation), simply ischemia 30 min without reperfusion (I group), reperfusion following ischemia 30 min (I/R group), 1 hour reperfusion following ischemia 30 min (I/R 1h group), 2 hours reperfusion following ischemia 30 min (I/R 2 h group) and 4 hours reperfusion following ischemia 30 min (I/R 4 h group). TNF-α and ICAM-1 were detected by immunohistochemical staining in myocardial tissue. Results: The expression of TNF-α in liver tissue was significantly increased, especially in I/R 1 h group (P0.05), but there were not significant changes in I/R 2 h group and I/R 4 h group. The expression of TNF-α and ICAM-1 in myocardial tissue were similar . The expressionss of TNF-α presented a positive correlation in liver tissue,compared with in myocardial tissue (r=0.844,P0.01). And also there was a positive correlation between the expression of TNF-α and ICAM-1 in myocardial tissue (r=0.7544, P0.01). At the same time, it can be found that there was hepatic cell edema, inflammatory cellular infiltration, even cellular necrosis following the process of liver ischemia/reperfusion injury through observing HE staining slice and in myocardial tissue, there were inflammatory cellular infiltration, myocardial fibre tumefaction. Conclusions: The expression of TNF-α formed in liver tissue following the process of liver ischemia/reperfusion injury can lead to the increasing expression of TNF-α in myocardial tissue ,and the later promoted high changes of ICAM-1 in myocardial tissue. The results suggested that TNF-α and ICAM-1 involved in the forming of myocardial tissue injury after liver ischemia/reperfusion.

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What this paper is about

Objective: To observe the expression of tumor necrosis factor-α (TNF-α)and intercellular adhesion molecule-1 (ICAM-1)induced by liver ischemia/reperfusion injury in Wistar rats, and study its mechanism. Methods: A model of liver ischemia/reperfusion injury was established by imitation. Forty-eight male Wistar rats were randomly divided into 6 groups as follows: the control group (the group of sham operation), simply ischemia 30 min without reperfusion (I group), reperfusion following ischemia 30 min (I/R group), 1 hour reperfusion following ischemia 30 min (I/R 1h group), 2 hours reperfusion following ischemia 30 min (I/R 2 h group) and 4 hours reperfusion following ischemia 30 min (I/R 4 h group). TNF-α and ICAM-1 were detected by immunohistochemical staining in myocardial tissue. Results: The expression of TNF-α in liver tissue was significantly increased, especially in I/R 1 h group (P0.05), but there were not significant changes in I/R 2 h group and I/R 4 h group. The expression of TNF-α and ICAM-1 in myocardial tissue were similar . The expressionss of TNF-α presented a positive correlation in liver tissue,compared with in myocardial tissue (r=0.844,P0.01). And also there was a positive correlation between the expression of TNF-α and ICAM-1 in myocardial tissue (r=0.7544, P0.01). At the same time, it can be found that there was hepatic cell edema, inflammatory cellular infiltration, even cellular necrosis following the process of liver ischemia/reperfusion injury through observing HE staining slice and in myocardial tissue, there were inflammatory cellular infiltration, myocardial fibre tumefaction. Conclusions: The expression of TNF-α formed in liver tissue following the process of liver ischemia/reperfusion injury can lead to the increasing expression of TNF-α in myocardial tissue ,and the later promoted high changes of ICAM-1 in myocardial tissue. The results suggested that TNF-α and ICAM-1 involved in the forming of myocardial tissue injury after liver ischemia/reperfusion.

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Available abstract

Objective: To observe the expression of tumor necrosis factor-α (TNF-α)and intercellular adhesion molecule-1 (ICAM-1)induced by liver ischemia/reperfusion injury in Wistar rats, and study its mechanism. Methods: A model of liver ischemia/reperfusion injury was established by imitation. Forty-eight male Wistar rats were randomly divided into 6 groups as follows: the control group (the group of sham operation), simply ischemia 30 min without reperfusion (I group), reperfusion following ischemia 30 min (I/R group), 1 hour reperfusion following ischemia 30 min (I/R 1h group), 2 hours reperfusion following ischemia 30 min (I/R 2 h group) and 4 hours reperfusion following ischemia 30 min (I/R 4 h group). TNF-α and ICAM-1 were detected by immunohistochemical staining in myocardial tissue. Results: The expression of TNF-α in liver tissue was significantly increased, especially in I/R 1 h group (P0.05), but there were not significant changes in I/R 2 h group and I/R 4 h group. The expression of TNF-α and ICAM-1 in myocardial tissue were similar . The expressionss of TNF-α presented a positive correlation in liver tissue,compared with in myocardial tissue (r=0.844,P0.01). And also there was a positive correlation between the expression of TNF-α and ICAM-1 in myocardial tissue (r=0.7544, P0.01). At the same time, it can be found that there was hepatic cell edema, inflammatory cellular infiltration, even cellular necrosis following the process of liver ischemia/reperfusion injury through observing HE staining slice and in myocardial tissue, there were inflammatory cellular infiltration, myocardial fibre tumefaction. Conclusions: The expression of TNF-α formed in liver tissue following the process of liver ischemia/reperfusion injury can lead to the increasing expression of TNF-α in myocardial tissue ,and the later promoted high changes of ICAM-1 in myocardial tissue. The results suggested that TNF-α and ICAM-1 involved in the forming of myocardial tissue injury after liver ischemia/reperfusion.

Key concepts: Ischemia, Reperfusion injury, Medicine, Tumor necrosis factor alpha, Liver tissue, Necrosis, ICAM-1, Immunohistochemistry

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The expression of TNF-α and ICAM-1 in myocardial tissue after liver ischemia-reperfusion in rats — Research Paper | ScholarLens