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2000•Journal of Forensic MedicineRequires access

Observation on early myocardial ischemia reperfusion injury with immunohistochemical method

Xiaohua Wu

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Abstract

In the present study,to investigate changes of the expression of c fos proto oncogene induced in early myocardial ischemia/reperfusion(MI/R),and we have established successfully the model of MI/R in 12 anaethetized SD rats. Parallelly,other 20 rats were used as normal and myocardial ischemia controls. After 30min ischemia followed by 30min reperfusion,the area of MI/R showed weak staining of cardiac myocytes(37 76±9 66) in part of cell nuclei,but after 60min ischemia followed by 30min reperfusion,staining of nuclei of cardiac myocytes(40 34±3 32) was stronger. The myocardium in normal and ischemia controls showed negative staining.No changes were seen with HE staining in all groups. The data indicates that the immunohistochemical method may reveal injury of MI/R experimentally.

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What this paper is about

In the present study,to investigate changes of the expression of c fos proto oncogene induced in early myocardial ischemia/reperfusion(MI/R),and we have established successfully the model of MI/R in 12 anaethetized SD rats. Parallelly,other 20 rats were used as normal and myocardial ischemia controls. After 30min ischemia followed by 30min reperfusion,the area of MI/R showed weak staining of cardiac myocytes(37 76±9 66) in part of cell nuclei,but after 60min ischemia followed by 30min reperfusion,staining of nuclei of cardiac myocytes(40 34±3 32) was stronger. The myocardium in normal and ischemia controls showed negative staining.No changes were seen with HE staining in all groups. The data indicates that the immunohistochemical method may reveal injury of MI/R experimentally.

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Available abstract

In the present study,to investigate changes of the expression of c fos proto oncogene induced in early myocardial ischemia/reperfusion(MI/R),and we have established successfully the model of MI/R in 12 anaethetized SD rats. Parallelly,other 20 rats were used as normal and myocardial ischemia controls. After 30min ischemia followed by 30min reperfusion,the area of MI/R showed weak staining of cardiac myocytes(37 76±9 66) in part of cell nuclei,but after 60min ischemia followed by 30min reperfusion,staining of nuclei of cardiac myocytes(40 34±3 32) was stronger. The myocardium in normal and ischemia controls showed negative staining.No changes were seen with HE staining in all groups. The data indicates that the immunohistochemical method may reveal injury of MI/R experimentally.

Key concepts: Ischemia, Immunohistochemistry, Myocyte, Staining, Myocardial ischemia, Reperfusion injury, Medicine, H&E stain

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