Establishment of human gastric epithelial cells carried BARF1 gene

The purpose of this study was to establish human gastric epithelial cells carried BARF1 gene,and construction in vitro model for studying the role of BARF1 gene in proliferation of gastric epithelial cells.The eukaryotic vector pcDNA3.1(+)-his/BARF1 for carried BARF1 gene was constructed and transfected into human gastric epithelial cell line GES-1,monoclonal was selected by G418.The proliferation status of gastric epithelial cells in untransfected gene BARF1,transfected empty vector and transfected BARF1 gene were detected with CCK-8.666bp products of BARF1 had been correctly connected to the eukaryotic expression vector pcDNA3.1(+)-his through double enzyme digestion.Obtained human gastric epithelial cells of stable expression BARF1 gene by G418 was selected.The proliferation of GES-1 cells transfected BARF1 gene was significantly higher than that of untransfected gene BARF1 and transfected empty vector(P0.01).Results indicated that human gastric epithelial cells carried BARF1 gene were established successfully.