ScholarLens Back to search
2013•Unpublished venueRequires access

Effect of Bcl-2 expression for BARF1 gene transfected into human gastric epithelial cells

Shuying Li

Open publisher page 0 citations

Abstract

Objective To investigate effect of Bcl-2 expression for BARF1 gene transfected into human gastric epithelial cells.Methods BARF1 gene carried by eukaryotic vector PIRES2-EGFP/BARF1 was transfected into human gastric epithelial cell GES-1,monoclonal was screened by G418.Bcl-2 was detected by Immunohistochemistry in each group(including non-transfected GES-1,transfected with empty vector GES-1 and BARF1 gene transfected GES-1).Results The human gastric epithelial cells of stable expression BARF1 gene was obtained.The expression of Bcl-2 was higher for the GES-1 cells of transfected BARF1 gene than those of untransfected GES-1 and transfected with empty vector GES-1.Conclusion The expression of Bcl-2 may be regulated in human gastric epithelial cells of BARF1 gene transfected.

About this research paper

What this paper is about

Objective To investigate effect of Bcl-2 expression for BARF1 gene transfected into human gastric epithelial cells.Methods BARF1 gene carried by eukaryotic vector PIRES2-EGFP/BARF1 was transfected into human gastric epithelial cell GES-1,monoclonal was screened by G418.Bcl-2 was detected by Immunohistochemistry in each group(including non-transfected GES-1,transfected with empty vector GES-1 and BARF1 gene transfected GES-1).Results The human gastric epithelial cells of stable expression BARF1 gene was obtained.The expression of Bcl-2 was higher for the GES-1 cells of transfected BARF1 gene than those of untransfected GES-1 and transfected with empty vector GES-1.Conclusion The expression of Bcl-2 may be regulated in human gastric epithelial cells of BARF1 gene transfected.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To investigate effect of Bcl-2 expression for BARF1 gene transfected into human gastric epithelial cells.Methods BARF1 gene carried by eukaryotic vector PIRES2-EGFP/BARF1 was transfected into human gastric epithelial cell GES-1,monoclonal was screened by G418.Bcl-2 was detected by Immunohistochemistry in each group(including non-transfected GES-1,transfected with empty vector GES-1 and BARF1 gene transfected GES-1).Results The human gastric epithelial cells of stable expression BARF1 gene was obtained.The expression of Bcl-2 was higher for the GES-1 cells of transfected BARF1 gene than those of untransfected GES-1 and transfected with empty vector GES-1.Conclusion The expression of Bcl-2 may be regulated in human gastric epithelial cells of BARF1 gene transfected.

Key concepts: Transfection, Molecular biology, Gene, Immunohistochemistry, Expression vector, Gene expression, Biology, Cell

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of Bcl-2 expression for BARF1 gene transfected into human gastric epithelial cells — Research Paper | ScholarLens