Relationship between expression of survivin gene and NET-1 protein in bladder carcinoma

Objective: To investigate the relationship between the expression of survivin gene and NET-1 protein in bladder carcinoma. Methods: By reverse transcriptase-polymerase chain reaction(RT-PCR) and immunohistochemical assay(SP), the expression of survivin gene and NET-1 protein were detected in 10 cases of normal tissues and 27 cases of bladder carcinoma tissues. The results were analyzed by corresponding statistica methods. Results: Survivin gene and NET-1 protein showed higher expression in bladder carcinoma. The rate of positive expressions were 62.96% and 77.78%, while the positive rates were 0% and 10% in nomal tissues. The difference was of significance between the two tissues(P0.01). The expression of survivin gene correlated with NET-1 protein expression(χ2=7.09, P0.01). The expressions of survivin gene and NET-1 protein were correlated with WHO pathology classification,TNM stage and prognosis(P0.01), and were incorrelated with age and sex. Conclusions: Both survivin gene and NET-1 protein were highly expressed in bladder carcinoma, which suggests that the ocurrence of bladder carcinoma occur was correlated with cellular apoptosis and proliferation. Survivin gene might play roles for clinical early diagnosis and target treatment of bladder carcinoma.