Comparative Study on Rapid Technology of Lonicera japonica Thunb.

[Objective] The research aimed to study the rapid technology of Lonicera japonica Thunb..[Method] The shoot tip and the stem segment of Lonicera japonica Thunb.were chose as explant,and surface sterilization,inoculation and cultivation were made to obtain the best material parts and the corresponding disinfection method.[Result] During the early culture,the growing status was better when the shoot tip was cultured in the medium adding to the 0.5-1.0 mg/L 6-BA,0-0.3 mg/L NAA or 0.1 mg/L IBA.When taking the stem segment as explant,callus were induced.During the Subculture,the medium adding to 0.5 mg/L 6-BA+0.10 mg/L IBA was the best.During the rooting culture,1/2 MS+ 1.5 mg/L IBA was the best.[Conclusion] A more rapid technology of superior varieties of Lonicera japonica Thunb.program was selected for the production of vaccine used to provide a more rapid reproduction and scientific way.