Study on Tissue Culture of Lonicera japonica Thunb. 'Jinfeng-1'

We studied the in vitro culture systematically on lonicera joponica of this eximious varietal 'Jinfeng-1' for the first time.The results showed that dip the apical buds with two leaves from farmland in the ethanol of 75% for 30 s at first,then in the HgCl2 of 0.1% for 15 minutes,and inoculated on MS+6-BA 1.0 mg/L+NAA 0.2 mg/L after washed in the germfree water for 5~6.According to these steps,we could get plantlets and the yield of vaccine was up to 78.90%;The best medium for rapid propagation of Lonicera japonica Thunb.was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+biotin-D 2 mg/L with agar in can bottle of 500 mL,and the propagation coefficient could reach 7.37 by 28 days.The best rooting medium was 1/2MS+IBA 3 mg/L,and the rooting rate could reach to 100%.