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Rapid Propagation in Vitro of Lonicera japonica Thunb.Clones

Jiang Xiangmei

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Abstract

With the stem segments as explants of superior clone Lonicera japonica Thunb.of Cangyuan 1#,the rapid propagation techniques in vitro were developed in this paper.The results showed that the optimum medium of shoot induction was MS +BA 0.5 mg/L+ IBA 0.5 mg/L,and the middle stem section was the suitable material for the explants;the appropriate proliferation medium was MS + BA 0.1~0.5 mg/L + NAA 0.1~0.3,the multiplication coefficient can reach 6.5;and the effective medium for rooting was MS+IBA 2.0 mg/L,the rooting rate can reach 93%.

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What this paper is about

With the stem segments as explants of superior clone Lonicera japonica Thunb.of Cangyuan 1#,the rapid propagation techniques in vitro were developed in this paper.The results showed that the optimum medium of shoot induction was MS +BA 0.5 mg/L+ IBA 0.5 mg/L,and the middle stem section was the suitable material for the explants;the appropriate proliferation medium was MS + BA 0.1~0.5 mg/L + NAA 0.1~0.3,the multiplication coefficient can reach 6.5;and the effective medium for rooting was MS+IBA 2.0 mg/L,the rooting rate can reach 93%.

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Available abstract

With the stem segments as explants of superior clone Lonicera japonica Thunb.of Cangyuan 1#,the rapid propagation techniques in vitro were developed in this paper.The results showed that the optimum medium of shoot induction was MS +BA 0.5 mg/L+ IBA 0.5 mg/L,and the middle stem section was the suitable material for the explants;the appropriate proliferation medium was MS + BA 0.1~0.5 mg/L + NAA 0.1~0.3,the multiplication coefficient can reach 6.5;and the effective medium for rooting was MS+IBA 2.0 mg/L,the rooting rate can reach 93%.

Key concepts: Explant culture, Japonica, clone (Java method), In vitro, Shoot, Botany, Murashige and Skoog medium, Biology

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