The Expression and Significance of HMGB1 in Rats with Acute Necrotizing Pancreatitis-associated Lung Injury

Objective:To investigate the role and the passible mechanism of HMGB1 in acute necrotizing pancreatitis-associated lung injury in rats.Methods:One hundred Sprague-Dawley rats were randomly divided into two groups: sham operation group(SO group)and acute necrotizing pancreatitis group(ANP group).Animals in each group were sacrificed at 6 h,12 h,24 h,36 h and 48 h af-ter operations respectively.The concentrations of serum HMGB1 were determined by enzyme-linked immunosorbent assay(ELISA).Blood gas analysis was performed.Moreover,pancreatic and pulmonary pathomorphology was compared between the two groups.Re-sults:In the ANP group,serum HMGB1 increased markedly at 6 h,and culminated at 48 h.There was a significant difference in serum HMGB1 concentrations between the ANP group and the SO group(P0.01).The severity of lung injury was parallel with the severity of pancreas injury.Conclusions:The extent of serum HMGB1 increase is positively correlated with the severity of ALI.