Expression and the Possible Effects of TREM-1 and HMGB-l in Rat Lung Tissue with Severe Acute Pancreatitis

Objective To investigate the expression and the possible effects of triggering receptor expressed on myeloid cells-1(TREM-1) and high mobility group box-l protein(HMGB-l) in rat lung tissue with severe acute pancreatitis.Methods 72 male SD rats were randomly divided into two groups:control group(group A) and severe acute pancreatitis model group(group B).Severe acute pancreatitis animal model was induced by injecting of 5% sodium taurocholate into biliopancreatic ducts retrogradely with fine needle.Rats in each group were sacrificed at 6 h,12 h,24 h,after operations respectively.The pathological changes of lung were observed by light microscopy,the ratio of wet weight to dry weight of lung was detected.The concentrations of TNF-α、IL-1β and HMGB-1 were measured by the method of ELISA.The expression of TREM-1 mRNA of lung tissue was detected by RT-PCR.Results Compared with group A,the extent of lung damage was severer and it lasted a growing trend in group B,the water content of lung tissue in group B was increased.Simultaneously,the levels of TNF-α、IL-1β、HMGB-1 and the expression of TREM-1 mRNA of lung tissue in group B were significantly higher,and the levels of TREM-1 mRNA and HMGB-1 were heightened duratively(P 0.05).Conclusion The levels of TREM-1 mRNA and HMGB-1 are positively correlated with the severity of severe acute pancreatitis associated lung injury.