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Determination of Icariin in Qianlie Shule Capsules by HPLC

Li Yong

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Abstract

Objective:To establish a method to determine the content of Icariin in Qianlie Shule Capsules. Methods: C 18 column was used with acetonitrile-water(23:77) as mobile phase and the detection wavelengthe was set at 270?nm. Results: The linear range was 0 092~1 84?μg for the Icariin (r=0 9997). The average recovery was 99 3% and RSD was 1 07% (n=6)?Conclusion: The method is simple and accurate, with a good reproducibility and can be used as a quantitative analysis method for this preparation.

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What this paper is about

Objective:To establish a method to determine the content of Icariin in Qianlie Shule Capsules. Methods: C 18 column was used with acetonitrile-water(23:77) as mobile phase and the detection wavelengthe was set at 270?nm. Results: The linear range was 0 092~1 84?μg for the Icariin (r=0 9997). The average recovery was 99 3% and RSD was 1 07% (n=6)?Conclusion: The method is simple and accurate, with a good reproducibility and can be used as a quantitative analysis method for this preparation.

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Available abstract

Objective:To establish a method to determine the content of Icariin in Qianlie Shule Capsules. Methods: C 18 column was used with acetonitrile-water(23:77) as mobile phase and the detection wavelengthe was set at 270?nm. Results: The linear range was 0 092~1 84?μg for the Icariin (r=0 9997). The average recovery was 99 3% and RSD was 1 07% (n=6)?Conclusion: The method is simple and accurate, with a good reproducibility and can be used as a quantitative analysis method for this preparation.

Key concepts: Icariin, Chromatography, Reproducibility, High-performance liquid chromatography, Chemistry, Linear range, Quantitative analysis (chemistry), Acetonitrile

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