Surveillance of antimicrobial resistance in Anhui Provincial Children's Hospital during 2011-2012

Objective To investigate the resistance of clinical bacterial isolates to commonly used antimicrobial agents in a hospital during 2011-2012.Methods Antimicrobial susceptibility testing of clinically isolated pathogens was performed by Kirby-Bauer method.Results A total of 3 946 pathogenic isolates were detected,gram-positive bacteria,gram-negative bacteria,fungi and mycoplasma accounted for 43.92%,37.91%,15.91%,and 2.26%respectively.The top one isolate was Escherichia coli(16.32%),followed by Streptococcus pneumoniae(16.02%),Staphylococcus aureus(9.00%),and Klebsiella pneumoniae(7.40%) and so on.Methicillin-resistant Staphylococcus aureus(MRSA) and methicillin-resistant coagulase-negative staphylococcus(MRCNS) respectively accounted for 41.41%(147/355) of Staphylococcus aureus and 81.40%(197/242) of coagulase-negative staphylococcus;extended-spectrum β-lactamase-producing rate of Escherichia coli and Klebsiella pneumoniae was 68.17%(439/644) and 72.95%(213/292) respectively.The resistant rate of Streptococcus pneumoniae to erythromycin and clindamycin was 99.84%and98.58%respectively;the resistance rate of MRSA to clindamycin and erythromycin was higher(75%),the resistance rates of MRSA and MRCNS to vancomycin,teicoplanin and linezolid were all 100.00%;the resistance rate of Enterococcus faeciumto vancomycin was 98.31%;the resistance rate of Acinetobacter baumannii to cefoperazone/sulbactam was low(8.70%),while to the other antimicrobial agents were all high;the resistance rate of Pseudomonas aeruginosa to imipenem,meropenem,ceftazidime,amikacin and ciprofloxacin were all low(26%).Four carbapenem-resistant ESBL-producing Klebsiella pneumoniae isolates and 15 pandrug-resistant Acinetobacter baumannii were detected.Conclusion Regular monitor on drug resistance of bacteria helps to understand the changes in bacterial resistance in hospitals,and provide the basis for empirical antimicrobial use.