Simultaneous Determination of Chlorogenic Acid,Quercetin and Kaempferol in Loquat Leaves by Non-ionic Surfactant Extraction Solvent Followed by High Performance Liquid Chromatography

Objective:An HPLC method for the simultaneous determination of chlorogenic acid,quercetin and kaempferol in loquat leaves was proposed based on rapid ultrasonic-assisted extraction using the non-ionic surfactant Triton X-100 as the extractant.Methods:The chromatographic separation was achieved on a Phenomenex C18 column using a mobile phase made up of methanol and 0.25% acetic acid at a flow rate of 1.0 mL/min,and the analytes were detected with a UV detector at 350 nm.Results:The calibration curves for chlorogenic acid,quercetin and kaempferol were linear in the range of 0.011-220μg/mL(r=0.9997),0.065-130μg/mL(r=0.9999),0.068-136μg/mL(r=0.9999),respectively.The limit of detection(LOD) was 1.94,0.22,0.27 ng/mL(RSN=3),respectively.The average recoveries across three spike levels ranged from 90.71% to 94.88%(n=3).Conclusion:The method is simple,rapid,convenient,sensitive,and environment-friendly,and thus can provide a new and scientific means for assaying the contents of flavonoids and chlorogenic acid in loquat leaves.