Assaying Hyperoside in Pill of Shanzha Tiaozhong with HPLC

Objective:To establish hyperoside assaying process with HPLC for quality control of Pill Shanzha Tiaozhong(PST).Method:HPLC was adopted.Chromatographic condition was Hypersil ODS C18(250×4.6 mm,5 μm).Mobile phase was acetonitrile-methanol-tetrahydrofuran-0.5% glacial acetic acid(AN-MeOH-THF-0.5% glacial acetic acid)(1:1:19.6:78.4).Flow rate was 0.8 mM/min.Detecting wavelength was 363 nm.Sample size was test article 5 μL and control article 20 μL.Calculating Cont.hyperoside was with external reference method.Result:The linear range of hyperoside was 10.84~97.56 μg,regression equation was Y=31 839X-13 664,R=0.999 8.The results of hyperoside assaying in three batches of PST were 50.378 3 μg,53.574 1 μg and 48.031 2 μg.Conclusion:The process is reliable and convenient for preparation and precise for separation and non-interference of other compositions.It suggests that the ingredient is specific and unique in detection under this condition,and the process can be as one of effective methods of assaying hyperoside for quality control of PST.