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2013•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of the contents of hyperoside and quercetin in Jiaoshanzha by HPLC

Lili Sun

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Abstract

Objective To establish an HPLC method to determine simultaneously hyperoside and quercetin in Jiaoshanzha. MethodsUse the HPLC,selecte phenomenon ODS C18 column(4.6mm × 250mm,5μm),acetonitrile-0.3% phosphoric acid as mobile phase gradient elution. ResultsThe average recoveries of hyperoside and quercetin were 98.8% and 100.1%,with RSD of 2.69% and 2.05%(n=6),respectively. ConclusionThis method is stable,accurate,and reliable to provides a scientific basis of quality control standard for the Jiaoshanzha.

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Objective To establish an HPLC method to determine simultaneously hyperoside and quercetin in Jiaoshanzha. MethodsUse the HPLC,selecte phenomenon ODS C18 column(4.6mm × 250mm,5μm),acetonitrile-0.3% phosphoric acid as mobile phase gradient elution. ResultsThe average recoveries of hyperoside and quercetin were 98.8% and 100.1%,with RSD of 2.69% and 2.05%(n=6),respectively. ConclusionThis method is stable,accurate,and reliable to provides a scientific basis of quality control standard for the Jiaoshanzha.

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Available abstract

Objective To establish an HPLC method to determine simultaneously hyperoside and quercetin in Jiaoshanzha. MethodsUse the HPLC,selecte phenomenon ODS C18 column(4.6mm × 250mm,5μm),acetonitrile-0.3% phosphoric acid as mobile phase gradient elution. ResultsThe average recoveries of hyperoside and quercetin were 98.8% and 100.1%,with RSD of 2.69% and 2.05%(n=6),respectively. ConclusionThis method is stable,accurate,and reliable to provides a scientific basis of quality control standard for the Jiaoshanzha.

Key concepts: Hyperoside, Quercetin, Phosphoric acid, Chromatography, High-performance liquid chromatography, Gradient elution, Chemistry, Biochemistry

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