Flow injection chemiluminescence determination of uric acid with micropipe-chip

Objective:To establish a flow injection chemiluminescence determination method with micropipe-chip for speedy determination of uric acid in clinical samples.Methods:Basing on the rule that uric acid can greatly enhance the chemiluminescence emission intensity of K4Fe(CN)6-K3Fe(CN)6-luminol system,the prepared micropipe-chip was used to analyze the uric acid in serum under the selective conditions.Results:The enhancing chemiluminescence intensity was proportional to uric acid concentration over a range 2.0×10-5～1.0×10-2 g/L.The detection limit(3σ) was 4.6×10-7 g/L.The relative standard deviation(RSD) was 3.09% and the recovery test results were 94.0%,99.7%,and 107.8%.Conclusion:The manufacture technique of micropipr-chip is simple but the proposed determination method of uric acid in human serum is sensitive,fast and convenient.