Influence of lipopolysaccharide on the proliferation of normal human skin fibroblasts

Objective To investigate the influence of lipopolysaccharide(LPS) on the proliferation of normal human skin fibroblasts and its relation with wound healing.Methods Normal skin fibroblasts were exposed to LPS for a certain period of time.Then proliferation of fibroblasts was determined with the colormetric thiazolylblue(MTT) assay and cell counting.Results The proliferation of normal skin fibroblasts increased when challenged with LPS at the concentration of 0.005 μg/mL,and the increase showed a concentration dependent manner.However,when the concentration of LPS was 0.5 μg/mL,the influence of LPS on the proliferation of normal skin fibroblasts began to decrease;When the concentration of LPS reached 1.0 μg/mL,the proliferation of fibroblasts was inhibited.[WTHZ]Conclusion LPS could promote the proliferation of normal human skin fibroblasts within a certain extent,suggesting that LPS might play an important role in wound healing.