Effects of Medium and External Hormone on Inducing,Gowth and Clorophyll Cntent of Ginkgo biloba Callus
Wang Wu
Abstract
Wang Wu
Abstract
In this manuscript,the Ginkgo biloba embryo was used as explant for callus induction experiment.Calli were induced from embryos on MS,WS and White medium supplied with various hormone combinations of 2,4-D,NAA,KT and BA.The induction,growth and chlorophyll content of Ginkgo biloba callus were systematically studied.The results listed as follows: 1) MS medium was the best inducing medium for the Ginkgo biloba embryos;2) The effects of external hormone on chlorophyll content were different and the order is KT,6-BA,NAA and 2,4-D;3) 1/4 MS medium supplied with NAA 2 mg/L and KT 3 mg/L was the best medium for its highest chlorophyll content.
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In this manuscript,the Ginkgo biloba embryo was used as explant for callus induction experiment.Calli were induced from embryos on MS,WS and White medium supplied with various hormone combinations of 2,4-D,NAA,KT and BA.The induction,growth and chlorophyll content of Ginkgo biloba callus were systematically studied.The results listed as follows: 1) MS medium was the best inducing medium for the Ginkgo biloba embryos;2) The effects of external hormone on chlorophyll content were different and the order is KT,6-BA,NAA and 2,4-D;3) 1/4 MS medium supplied with NAA 2 mg/L and KT 3 mg/L was the best medium for its highest chlorophyll content.
Key concepts: Ginkgo biloba, Callus, Ginkgo, Explant culture, Chemistry, Murashige and Skoog medium, Botany, Ginkgoales