Study on the Optimum Combinations of Plant Hormone in Inducing Callus of Ginkgo Biloba L.
Shang Fu
Abstract
Shang Fu
Abstract
The optimum combination of hormone, and the other closely related factors in inducing callus of Ginkgo biloba L. were systematically studied so as to obtain the fresh, power_vitality and great_quantity callus for the further suspension culture. The result indicated that the most appropriate medium was N6 supplemented with 3 0?mg/L NAA and 1 0 mg/L BAl, the advantage and concentration of NAA, 2,4-D, GA ,BA, KT varied greatly, the combination NAA with BA was suitable for both culture and subculture, 2,4-D was suitable for inducing, GA was not, KT was beneficial for differentiation of callus.
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The optimum combination of hormone, and the other closely related factors in inducing callus of Ginkgo biloba L. were systematically studied so as to obtain the fresh, power_vitality and great_quantity callus for the further suspension culture. The result indicated that the most appropriate medium was N6 supplemented with 3 0?mg/L NAA and 1 0 mg/L BAl, the advantage and concentration of NAA, 2,4-D, GA ,BA, KT varied greatly, the combination NAA with BA was suitable for both culture and subculture, 2,4-D was suitable for inducing, GA was not, KT was beneficial for differentiation of callus.
Key concepts: Callus, Ginkgo biloba, Subculture (biology), Suspension culture, Botany, Tissue culture, Ginkgo, Chemistry