Effect of 8-bromo-7-methoxychrysin(BrMChR)on proliferation and inducing apoptosis in human small cell lung cancer cell line

Objective To observe the effect of 8-bromo-7-methoxychrysin(BrMChR) on proliferation and inducing apoptosis in human small cell lung cancer(SCLC) cell line NCI-H446.Methods The inhibitive effect of method was used to test inhibition effect of BrMChR on clone formation of NCI-H446 cells.DNA ladder bands were observed by DNA agarose gel electrophoresis.Cell cycle phase was detected by flow cytometry(FCM) with PI staining.Results MTT test showed the proliferation of NCI-H446 cell was strongly inhibited by BrMChR in a dose-dependent manner.BrMChR could inhibit clone formation of NCI-H446 cells.DNA fragment assay indicated that DNA ladder bands could be shown in DNA agarose gel electrophoresis after treatment with BrMChR of different concentration for 48h.FCM analysis indicated cell cycle phase was blocked by BrMChR in G1 phase.Conclusion BrMChR can inhibit the proliferation of NCI-H446 cells and induce apoptosis.