Determination of proanthocyanidins content in Pyracantha fortuneana fruit

Vanillin-HCl assay was adopted to determine the content of proanthocyanidins in Pyracantha fortuneana extract.Several parameters,such as standard,light,reaction time,reaction temperature,HCl concentration and vanillin concentration that affecting the color reaction of proanthocyanidin were investigated.The results showed the appropriate reaction conditions were as the following,1mL of pyracantha extract,6mL of 4g/100mL vanillin solution and 3mL of concentrated HCl in methanol respectively,after blending the reaction was carried out at 30℃ for 10min and then measured the absorbance at 500nm.Assessment of the method by statistics showed the method has good repeatability,stability,reproducibility and high recovery rate.