Inhibition effect and mechanism of sodium butyrate on human gastric carcinoma cell line MKN-28 in vitro

Objective To investigate the effects and mechanism of histone deacetylase inhibitors sodium butyrate on cell growth inhibition in human gastric carcinoma cell MKN-28.Methods The inhibiting effects of cell growth was assayed by MTT method;cell cycles and apoptosis were analyzed by flow cytometry(FCM);The expression of apoptosis-related gene p21WAF1 Was detected by RT-PCR before and after sodium butyrate treateded.Results Sodium butyrate(1.0,2.5,5.0 mmol/L) all showed inhibitory effects on the proliferation of MKN-28cell in dose-and-time dependent manner(P0.05);The increasing G0/G1 stage cells and decreasing S phase cells were observed significantly at 72 h after NaB(1.0,2.5,5.0 mmol/L) treatment(P0.05),apoptosis rates were 13.7%±0.8%,20.8%±2.4%,33.6%±2.6% respectively,the difference is significantly when compare with the control groupe 2.8%±0.4%(P0.05).The level of p21WAF1 was higher in the sodium butyrate treated cell than the cell without treatment.Conclusion Sodium butyrate could inhibit growth of MKN-28 cells effectively.It may be related to p21WAF1 gene up-regulating,apoptosis inducing and G0/G1 blocking.