2012•Hubei nongye kexueRequires access

Analysis on the Genetic Relationship of Osmanthus fragrans Cultivars in Xianning with SRAP Markers

Lu Yuan

Abstract

The genetic relationship of 32 Osmanthus fragrans cultivars in Xianning was analyzed by sequence-related amplified polymorphism(SRAP).210 bands were amplified from 15 primer pairs,out of which 182 were polymorphic,accounting for 86.67%.The Shannon's index(0.339 3) and genetic diversity(0.216 4) within Albus group were the highest among 4 cultivar groups of O.fragrans.A high level of genetic differentiation(57.89%) among O.fragrans cultivar groups was detected based on Nei's genetic diversity analysis.According to the dendrogram constructed by UPGMA,the cultivars were classified into 5 groups at the level of genetic coefficient 0.644.The geographical distributions of the tested cultivars were correlated to their taxonomic positions.

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Key concepts: Osmanthus fragrans, UPGMA, Cultivar, Dendrogram, Genetic diversity, Biology, Genetic relationship, Botany