2014•Journal of Hubei UniversityRequires access

Analysis on the genetic diversity of Osmanthus fragrans Lour. in Xianning with ISSR markers

Fan Fuhu

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Abstract

Forty-eight O.fragrans Lour.accessions in Xianning Region were analyzed by ISSR markers.A total of 81bands were yield from the 11primers,which were screened based on their polymorphism,clearity and reproducibility.Among the obtained markers,70 were polymorphic, accounting for 86.4% of the total.Analyzed by NTSYSpc 2.10e,the genetic similarity coefficient among the accessions ranged from 0.41to 0.97,reflecting the high genetic diversity of O.fragrans Lour.in Xianning Region.The UPGMA dendrogram demonstrated a big genetic distances between Fragrans group,which bloomed from September to April of the following year,and Qiugui(Latifolius,Aurantiacus,Thunbergii)group,which bloomed 1—2times in September or October, illustrated that phylogenetic relationship of O.fragrans Lour.had a certain relevance with the habit of blossom.

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Forty-eight O.fragrans Lour.accessions in Xianning Region were analyzed by ISSR markers.A total of 81bands were yield from the 11primers,which were screened based on their polymorphism,clearity and reproducibility.Among the obtained markers,70 were polymorphic, accounting for 86.4% of the total.Analyzed by NTSYSpc 2.10e,the genetic similarity coefficient among the accessions ranged from 0.41to 0.97,reflecting the high genetic diversity of O.fragrans Lour.in Xianning Region.The UPGMA dendrogram demonstrated a big genetic distances between Fragrans group,which bloomed from September to April of the following year,and Qiugui(Latifolius,Aurantiacus,Thunbergii)group,which bloomed 1—2times in September or October, illustrated that phylogenetic relationship of O.fragrans Lour.had a certain relevance with the habit of blossom.

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Available abstract

Forty-eight O.fragrans Lour.accessions in Xianning Region were analyzed by ISSR markers.A total of 81bands were yield from the 11primers,which were screened based on their polymorphism,clearity and reproducibility.Among the obtained markers,70 were polymorphic, accounting for 86.4% of the total.Analyzed by NTSYSpc 2.10e,the genetic similarity coefficient among the accessions ranged from 0.41to 0.97,reflecting the high genetic diversity of O.fragrans Lour.in Xianning Region.The UPGMA dendrogram demonstrated a big genetic distances between Fragrans group,which bloomed from September to April of the following year,and Qiugui(Latifolius,Aurantiacus,Thunbergii)group,which bloomed 1—2times in September or October, illustrated that phylogenetic relationship of O.fragrans Lour.had a certain relevance with the habit of blossom.

Key concepts: Osmanthus fragrans, Genetic diversity, UPGMA, Dendrogram, Biology, Phylogenetic tree, Genetic similarity, Botany

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