Genetic Relationship and Specific Markers of Some Osmanthus fragrans Cultivars Based on ISSR

Interspecific relationships of 81 cultivars and 1 wild species of Osmanthus fragrans Lour.were evaluated by using ISSR marker,with Osmanthus heterophyllus(G.Don.) P.S.Green and Osmanthus cooperi Hemsl.as contrast species.28 primers produced 280 loci,out of which 263 were polymorphic.The percentage of polymorphic loci was 93.93%.The Shannon information index(0.370 7) and genetic diversity(0.241 7) of Albus Group were the highest among 4 groups of Osmanthus fragrans.The measurement of genetic variation showed that the coefficient of genetic differentiation of Osmanthus fragrans was 57.39%.Most of the genetic variation existed among groups.The cluster analysis showed that four groups could be clustered when genetic similarity coefficient was given as 0.71.The genetic distance between Asiaticus Group and other 3 groups was far.Cultivars with deep color in Luteus Group often cluster together with cultivars in Aurantiacus Group.The cluster result based on ISSR marker was approximately consistent with taxonomic result based on morphology.84 materials could be differentiated with combination of primer ISSR12 and ISSR27.