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2006•Journal of Henan University of Science & TechnologyRequires access

Optimum Conditions of Aspergillus Niger LH2446 Solid State Fermentation to Produce Cellulase

Wang Shi, Kai Hu

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Abstract

This study is carried out to explore the optimum conditions of Aspergillus niger LH2446 solid state fermentation to produce cellulase.It concludes that the optimum conditions are as follow: the proportion of straw to wheat bran is 3∶2,(NH_4)_2SO_4 is 2.0% and 10g media are filled in 250mL flask with normal pH,the surfactant Tween20 is 0.5%,3d culture time and 35℃ culture temperature.Under optimum conditions,LH2466 is able to accumulate an average of 19920U/g and a maximum of 20100U/g of cellulase.

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What this paper is about

This study is carried out to explore the optimum conditions of Aspergillus niger LH2446 solid state fermentation to produce cellulase.It concludes that the optimum conditions are as follow: the proportion of straw to wheat bran is 3∶2,(NH_4)_2SO_4 is 2.0% and 10g media are filled in 250mL flask with normal pH,the surfactant Tween20 is 0.5%,3d culture time and 35℃ culture temperature.Under optimum conditions,LH2466 is able to accumulate an average of 19920U/g and a maximum of 20100U/g of cellulase.

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Available abstract

This study is carried out to explore the optimum conditions of Aspergillus niger LH2446 solid state fermentation to produce cellulase.It concludes that the optimum conditions are as follow: the proportion of straw to wheat bran is 3∶2,(NH_4)_2SO_4 is 2.0% and 10g media are filled in 250mL flask with normal pH,the surfactant Tween20 is 0.5%,3d culture time and 35℃ culture temperature.Under optimum conditions,LH2466 is able to accumulate an average of 19920U/g and a maximum of 20100U/g of cellulase.

Key concepts: Aspergillus niger, Cellulase, Solid-state fermentation, Bran, Fermentation, Food science, Straw, Chemistry

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