Cellulase production condition and optimization by two mixed fungi Fermentation

The solid-state fermentation(SSF) method was introduced into the cellulase-producing ability analysis.The individual and mixed fermentation of two fungi X-13,X-6 were compared.The results showed that cellulase activity in the mixed fermentation increased by 31.0% and 40.7% respectively,compared to the individual fermentation.Single-factor test showed that,the optimum straw and nitrogen source were rice straw and(NH4)2SO4 respectively.The media amount was 5 g.The optimum temperature was 30℃ and the optimum time was 4~6 days.Response Surface Methodology(RSM) was used to optimize the fermentation condition of cellulase production by the two fungi mixed.The optimum fermentation condition was rice straw:bran 1∶2,(NH4)2SO4 3.65 g/L,the inoculum size 0.75 mL,stuff:water 1∶2.5,X-13∶X-6 2∶3,initial pH 5.8,tween-80 0.1%,cultural temperature 30℃,incubation time 6 days.Under the optimum condition,the cellulase activity reached 25.928 U/g,which was increased by 112.9% compared with the initial cellulase activity 12.181 U/g.