Cloning and expression analysis of GbMYB25gene from Gossypium barbadense

MYB transcription factors,as one of the largest gene families in plants,play an important role in multiple biological processes.According to the coding sequence of GhMYB25 gene,apair of primers were designed.The homologous sequence of GhMYB25 gene,designated GbMYB25,was cloned from cotton(Gossypium barbadense L.)cultivar Xinhai21 using reverse transcription PCR(RT-PCR)methods.GbMYB25 gene contained an open reading frame(ORF)of 930 bp in length encoding 309 amino acids residues with a predicted molecular mass of 34.762 ku and a basic isoelectric point of 8.08,with two highly conserved SANT domains in the encoded putative protein.Genomic DNA sequence analysis showed that GbMYB25 contained 2introns.Amino acid sequence alignment revealed that N-terminal of GbMYB25 shared high degree of identity with other higher plant MYB proteins.The phylogenetic tree showed that GbMYB25 was located at the same branch with GhMYB25.Subcellular localization showed that GbMYB25 expressed possibly in cell nucleus.The experiment results of real time PCR exhibited that GbMYB25 had a higher expression level in ovule(0doa),fiber(5dpa)and leaf.Above results suggested that GbMYB25 transcription factor might be involved in cotton fiber development.