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Content analysis of hesperidin in Pericarpium Citri Retiulatae from different species in Guangxi

Xiaowei Chen

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Abstract

Objective:To establish a fast and simple determination method for hesperidin content from five different species of Pericarpium Citri Retiulatae in Guangxi.Methods:The optimum extracting condition was optimized through orthogonal design test,and the hesperidin were separated and determined by TLC-UV.Results:The calibration curve showed a good linear relationship when hesperidin was in 2.7～27.0 μg/mL(r=0.9992,n=6).The hesperidin contents in the samples from different species were 24.45～30.87 mg/g.The results showed the hesperidin content in the sample from the peel of navel orange is highest.Conclusion:The method for the determination of hesperidin from Pericarpium Citri Reticulatae has high duplication and accuracy.It can be used for quality control of Pericarpium Citri Reticulatae.

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Objective:To establish a fast and simple determination method for hesperidin content from five different species of Pericarpium Citri Retiulatae in Guangxi.Methods:The optimum extracting condition was optimized through orthogonal design test,and the hesperidin were separated and determined by TLC-UV.Results:The calibration curve showed a good linear relationship when hesperidin was in 2.7～27.0 μg/mL(r=0.9992,n=6).The hesperidin contents in the samples from different species were 24.45～30.87 mg/g.The results showed the hesperidin content in the sample from the peel of navel orange is highest.Conclusion:The method for the determination of hesperidin from Pericarpium Citri Reticulatae has high duplication and accuracy.It can be used for quality control of Pericarpium Citri Reticulatae.

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Available abstract

Objective:To establish a fast and simple determination method for hesperidin content from five different species of Pericarpium Citri Retiulatae in Guangxi.Methods:The optimum extracting condition was optimized through orthogonal design test,and the hesperidin were separated and determined by TLC-UV.Results:The calibration curve showed a good linear relationship when hesperidin was in 2.7～27.0 μg/mL(r=0.9992,n=6).The hesperidin contents in the samples from different species were 24.45～30.87 mg/g.The results showed the hesperidin content in the sample from the peel of navel orange is highest.Conclusion:The method for the determination of hesperidin from Pericarpium Citri Reticulatae has high duplication and accuracy.It can be used for quality control of Pericarpium Citri Reticulatae.

Key concepts: Hesperidin, Chemistry, Linear relationship, Orange (colour), Chromatography, Food science, Horticulture, Mathematics

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Content analysis of hesperidin in Pericarpium Citri Retiulatae from different species in Guangxi — Research Paper | ScholarLens