Content Determination of Hesperidin from Different Kinds of Pericarpium Citri Reticulatae by HPLC

Objective:To determine the content of the hesperidin in six different kinds of pericarpium citri reticulatae and gained a method to estimate and control the quality of medical material and effectively.Method: The HPLC was adopted to do this examination,the separat on was performed on a Baseline C18 column(4.60 mm×250 mm,5 μm),methanol-water(1% acetic acid solution)(50∶50) was used as the mobile phase with isocratic elution,the detective wavelength was at 283 nm,the flow rate was 1.0 mL· min-1,the column temperature was room temperature and the injecti on volume was 20 μL.Result: The hesperidin showed a good linearity in the range of 0.002-0.040 g·L-1(r=0.999 9),the average recovery was 102.3%(n=9),RSD 1.6%.The contents of hesperidin in peels of citrus was varied from different species.Conclusion: The method is simple and available,which can be used to determine and estimate hesperidin in different kinds of pericarpium citri reticulatae.