Supercritical Carbon Dioxide Extraction of Lycopene from Blakeslea trispora

[Objective] The purpose of the study was to introduce the new extraction technology of lycopene from Blakeslea trispora.[Method] The lycopene was extracted from B.trispora by supercritical carbon dioxide extraction technology and the influence of extraction time,extraction temperature,extraction pressure and flow rate of carbon dioxide on the extraction rate was studied.[Result] After the filtered fermentation broth was pretreated with 95% alcohol,the extraction rate of lycopene was highest when the extraction time was 3 h;its extraction rate was highest when the extraction temperature was 45 ℃ and then its extraction rate was decreasing along with the ascending of temperature;the optimum extraction pressure was 45 MPa and the optimum flow rate of carbon dioxide was 20 L/h.[Conclusion] The optimum technology for extracting lycopene from B.trispora by supercritical carbon dioxide extraction technology was as follows: the pressure was 45 MPa,the temperature was 45 ℃ and the flow rate of carbon dioxide was 20 L/h.Under this condition,the yield rate of lycopene could reach 76%.